One of the things that I’m always curious about, is the effect of a sequence variation on the conformation of a target. Recently I wrote about ClinVar and SwissVar, two tools for examining variants and their associations with disease. Since the PDB tends to have either wild type versions of a protein, or versions of the protein docked with some molecule, I thought it might be interesting to look at the mutant form of a protein. Or at least what the predicted conformation of that protein might look like.
Expasy, the folks that brought you all SwissProt, have an interesting tool called SwissModel that lets you model a protein given a protein sequence. I was curious about KRASG12D, so I pasted the edited sequence into SwissModel, and this was the result.
Although, it’s not entirely visible from this screenshot, you can see the resulting models below.